The advantage of AlphaLISA is that it is a no-wash alternative to Enzyme Linked Immuno-Sorbant Assays (ELISAs) for higher throughput drug discovery. 

AlphaLISA is a bead-based immunoassay technology designed to detect analytes in biological samples. It is based on a chemi-luminescent readout ideally suited for miniaturisation, in our case 384 well. AlphaLISA assays have a wide dynamic range, are sensitive and are more robust compared to conventional ELISA assays.

The AlphaLISA technology relies on an amplified luminescence proximity homogenous assay and uses luminescent oxygen-channeling chemistry. 

AlphaLISA assays can be set up as a sandwich or competition assays. 

In a sandwich assay, the analyte is captured by a biotinylated antibody bound to a streptavidin-coated donor bead and a second antibody is conjugated to an acceptor bead. Upon binding of the two antibodies to the analyte, the donor and acceptor beads are brought into close proximity and a laser is used as the excitation source to excite the donor bead at 680nm. This generates a singlet oxygen and triggers a cascade of chemical reactions in the nearby acceptor bead, resulting in a chemi-luminescence signal at 615nm. 

By comparison, in the competitor assay format, a biotinylated analyte bound to a streptavidin donor bead is used and an antibody conjugated to the AlphaLISA acceptor bead.