Developed to perform quantitative optical screening for cell-based kinetic assays, Fluorescent Imaging Plate Reader (FLIPR) screening is used in a variety of assay formats, readouts and target types.
FLIPR Screening Assay Formats
We use FLIPR in a variety of assay formats, readouts and target types with a selection of examples listed below:
- Antagonist and agonist screening recombinant cell-based assays
- Cells expressing a target GPCR are loaded with calcium flux dye (Fluo-4) and treated with the GPCR ligand in the absence or presence of various compounds that bind to the GPCR and inhibit calcium response.
- Dose response curve generated in a HEK-293 cell line expressing a 7-Transmembrane (7-TMR) or G-Protein-Coupled Receptor (GPCR) in a 384 well assay format
- More recently, we have used our FLIPR Tetra, with the luminescent option, to successfully screen in 384 and 1536 well format using aqueorin cell lines purchased from PerkinElmer. We have experience of screening over a million compounds per assay in this format using ‘assay ready plates’ (first addition to the well is the compound).
- Phenotypic screening of 7-TM receptos in human primary cells
- Human neutrophils were isolated from donor blood, incubated with calcium flux dye, dispensed into 384 well plates and treated with increasing concentration of FMLF ligand.
- Screening of compounds in Human Neutrophil FLIPR antagonist assay in 1536 well plates
- Human neutrophils were isolated from the whole blood of donors by densite gradient centrifugation, loaded with Fluor-loading dye, dispensed into 1536 well plates incubated in the presence of compounds, and screened with agonist addition on-line.
Download our eBook to find out more about assay development:
“Following a lengthy search, we identified Gary, Kathy and team as the ideal partner to develop and screen a particularly difficult calcium flux assay based on isolated primary mitochondria. Their scientists developed a robust, reliable, 384 well assay that they then used to screen over 200,000 compounds. From this screen, we successfully identified hit compounds that were taken forward into Lead Optimisation.
The team were excellent to work with, delivering a high level of scientific competency and understanding of the target biology coupled with significant experience of the readout technology (FLIPR instrument).
I obtained consistent, information rich communication within the project, excellent data sharing, along with achieving the deliverables and milestones in a timely manner. The interaction was excellent, and my expectations were achieved, a real success and excellent value proposition. I would highly recommend them to anyone searching to outsource challenging bioscience projects. A truly “Science-led, employee-centric, research provider”.
The team were excellent to work with, delivering a high level of scientific competency and understanding of the target biology coupled with significant experience of the readout technology (FLIPR instrument).
I obtained consistent, information rich communication within the project, excellent data sharing, along with achieving the deliverables and milestones in a timely manner. The interaction was excellent, and my expectations were achieved, a real success and excellent value proposition. I would highly recommend them to anyone searching to outsource challenging bioscience projects. A truly “Science-led, employee-centric, research provider”.
