Bioanalysis Information Leaflet
Bioanalysis is the quantitative measurement of compounds in biological matrices.
At Charnwood Discovery, non-GLP bioanalysis is conducted on a range of sample sources such as cell based assays, pharmacokinetic (PK) and pharmacodynamic (PD) studies, dose range-finding and maximum tolerated dose (MTD) studies.
All data is checked independently by an experienced scientist to ensure acceptance criteria and data quality standards are met.
Standard Assay Conditions
- Experience processing plasma, serum, blood, urine, bile, tissues and tumours, as well as in vitro samples
- Tissues homogenised using the Precellys® Evolution Homogeniser with Cryolys® Evolution cooling unit to protect heat sensitive samples
- Separate standard and QC weighed to 0.01 mg
- Matrix-matched calibration curves
- Protein precipitation of samples/homogenized tissues
- Quantitative analysis by LC-MS
Figure 1: Left—Precellys® Evolution Homogenizer with Cryolys® Evolution cooling unit. Right—Plasma quenched with solvent.
Figure 2: Example calibration curve.
Deliverables
Excel summary of sample concentrations (ng/mL or ng/g), and LC-MS method.
Compound Requirements
Bioanalysis: 2 x 1 mg (weighed to 0.01 mg) or >6 mg solid.
