Kinetic Solubility

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Kinetic Solubility Assay Information

Aqueous solubility, the phenomenon of dissolution of a solute in a solvent, is one of the most important physicochemical parameters for any compound. Low solubility can cause problems in many different in vitro testing techniques leading to unreliable results and/or reproducibility problems.

Kinetic solubility is measured with a high throughput assay using a stock solution of compound already dissolved in an organic solvent (typically DMSO). This stock is added to buffer, mixed and any resultant precipitate post-incubation separated from the buffer by filtration using MultiScreen® Solubility filter plates with the filtered sample analyzed against a standard curve.

Standard Assay Conditions

  • 90 minute solubilization
  • 2% DMSO final in solubilization mixture
  • Buffer: PBS pH 7.4
  • N=2 replicates
  • Low, medium and high solubility controls included
  • Analysis by UV absorption
    • Analysis by LC-MS is also available if no chromophore is present
  • 5-200 µM reportable range
Kinetic Solubility

Example Standard Curve

Kinetic-Solubility

Compound Requirements

100 µL of a 10 mM stock or 1 mg of solid (accurately weighed to 2 decimal places)

Deliverables

Kinetic Solubility in µM

Download Technical Resource Here

In Vitro Assays

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