Kinetic Solubility Assay Information
Aqueous solubility, the phenomenon of dissolution of a solute in a solvent, is one of the most important physicochemical parameters for any compound. Low solubility can cause problems in many different in vitro testing techniques leading to unreliable results and/or reproducibility problems.
Kinetic solubility is measured with a high throughput assay using a stock solution of compound already dissolved in an organic solvent (typically DMSO). This stock is added to buffer, mixed and any resultant precipitate post-incubation separated from the buffer by filtration using MultiScreen® Solubility filter plates with the filtered sample analyzed against a standard curve.
Standard Assay Conditions
- 90 minute solubilization
- 2% DMSO final in solubilization mixture
- Buffer: PBS pH 7.4
- N=2 replicates
- Low, medium and high solubility controls included
- Analysis by UV absorption
- Analysis by LC-MS is also available if no chromophore is present
- 5-200 µM reportable range
Example Standard Curve
100 µL of a 10 mM stock or 1 mg of solid (accurately weighed to 2 decimal places)
Kinetic Solubility in µM