Chromatographic LogD

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Chromatographic LogD Assay Information

A compound’s lipophilicity provides some indication of how it may behave in the body. The traditional “shake flask” method is time consuming and not suitable for high throughput and reversed phase HPLC is acknowledged to be a useful alternative method to determine lipophilicity. 

Compound in DMSO is analysed by LC-UV-MS on a linear gradient with 10 calibration compounds of known Chromatographic Hydrophobicity Index (CHI) value.  A calibration curve is created relating CHI value to UV peak retention time and the CHI value determined.  CHI LogD and chromatographic LogD values are then calculated.

UV Chromatogram of the 10 Calibrants

Chromatogphic LogD
Chromatogphic LogD_calibration curve

Example calibration: CHI pH 7.4 plotted against retention time for the 10 compounds used as calibrants for the chromatographic LogD assay (right), chromatogram presented to the left.

Chromatogphic LogD_UPLC

Standard Assay Conditions

  • 0.5 mM compound in DMSO
  • Analysis by LC-UV-MS

Compound Requirements

10 µL of a 10 mM stock or 1 mg of solid (accurately weighed to 2 decimal places)

Deliverables

Chromatographic LogD and CHI LogD

Download Technical Resource Here

In Vitro Assays

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